Optimization of Laccase from Ganoderma lucidum Decolorizing Remazol Brilliant Blue R and Glac1 as Main Laccase-Contributing Gene
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چکیده
منابع مشابه
Purification and Characterization of Pleurotus florida Laccase (L1) involved in the Remazol Brilliant Blue R (RBBR) Decoloration
Pleurotus florida produces two extracellular laccase (L1 and L2) isoenzymes and the L1 isoenzyme is dominantly involved in the dye decoloration process. L1 isoenzyme was successfully purified to 6.4 fold with a yield of 36% and had a specific activity of 52.6 U mg of protein. The purified laccase was monomeric with an apparent molecular mass of ≈54 kDa. The optimum pH and temperature of the LI ...
متن کاملRemazol Brilliant Blue R Dye Decolourization by Laccase Produced by Pleurotus Sajor-caju via Solid-State Fermentation
The application of synthetic dyes in various industries improved the aesthetic properties of the products, but the discharge of dye-containing effluent into water bodies leads to serious environmental problems. Till date, colour removal from effluent is still a challenge due to the absence of economically attractive treatments. In this study, decolourization of a synthetic dye – Remazol brillia...
متن کاملProduction and Purification of Remazol Brilliant Blue R Decolorizing Peroxidase from the Culture Filtrate of Pleurotus ostreatus.
An extracellular H(inf2)O(inf2)-requiring Remazol brilliant blue R (RBBR) decolorizing enzymatic activity was found in the culture medium of Pleurotus ostreatus. The enzymatic activity was maximally obtained in idiophase, and the optimum C/N ratio was 24. High C/N ratios repressed the enzymatic activity, and addition of veratryl alcohol had no effect on the production of enzyme. The enzyme was ...
متن کاملOptimization of laccase production by two strains of Ganoderma lucidum using phenolic and metallic inducers.
Ganoderma lucidum (Curtis) P. Karst is a white rot fungus that is able to degrade the lignin component in wood. The ability of two strains of this species to produce the ligninolytic enzyme laccase was assessed. After the evaluation of induction with heavy metals and phenolic compounds, it was found that among the tested substances, copper and ferulic acid are the best laccase inducers. It was ...
متن کاملoptimization of dye removal from aqueous remazol brilliant blue r (rbbr) by trametessp. pellets
decolorization of remazol brilliant blue r (rbbr) by trametessp. pellets was studied in a batch reactor. dye removal process was performed in shaken flasks which contained 100 ml of rbbr aqueous solution and fungal pellets. the process was followed for 48 h and the dye removal was analyzed at a visible spectrum of 590 nm. response surface methodology (rsm) employing box behnken design at three ...
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ژورنال
عنوان ژورنال: Molecules
سال: 2019
ISSN: 1420-3049
DOI: 10.3390/molecules24213914